Lasunción M A, Herrera E
Arch Int Physiol Biochim. 1980 Oct;88(4):385-91. doi: 10.3109/13813458009092909.
Triglyceride-rich lipoproteins from adult rat plasma were labelled in vivo with 3H in the esterified fatty acids and 14C in the labelled glyceride glycerol of neutral lipids by injecting i.v. sodium 9-10 (n)-[3H] palmitate and [U-14C] glycerol, after which the prelabelled lipoproteins were purified by ultracentrifugation and dialysis. The lipoproteins were incubated in vitro, in the presence or not of heparin, with pieces of epididymal fat pads or isolated adipocytes from fed rats. The disappearance of both [3H]- and [14C] lipids from the media was greater when incubations were performed with adipocytes than with fat-pad pieces and it increased with heparin in both preparations. More 3H-label than 14C was found in the tissue lipids, a higher percentage being present in adipocytes than in fat-pad pieces, and the amount of label in tissue lipids was always enhanced by heparin. Some 14C-label appeared as esterified fatty acids in both tissue preparations and it also was enhanced by the presence of heparin. These findings are in agreement with the recognized influence of heparin on the release of lipoprotein lipase and show the direct relationship between heparin action and tissue ability to take up products of lipoprotein triglyceride breakdown. They also demonstrate the ability of adipose tissue to metabolize glycerol coming from the hydrolysis of lipoprotein glycerides.
通过静脉注射9-10（n）-[3H]棕榈酸钠和[U-14C]甘油，将成年大鼠血浆中富含甘油三酯的脂蛋白在体内用酯化脂肪酸中的3H和中性脂质标记甘油酯甘油中的14C进行标记，之后通过超速离心和透析对预先标记的脂蛋白进行纯化。将脂蛋白在体外与喂食大鼠的附睾脂肪垫碎片或分离的脂肪细胞一起孵育，有无肝素存在均可。与脂肪垫碎片相比，用脂肪细胞进行孵育时，培养基中[3H]和[14C]脂质的消失量更大，并且在两种制剂中，肝素均可使其增加。在组织脂质中发现的3H标记比14C更多，脂肪细胞中的百分比高于脂肪垫碎片，并且肝素总是会增加组织脂质中的标记量。在两种组织制剂中，一些14C标记以酯化脂肪酸的形式出现，并且肝素的存在也会使其增加。这些发现与肝素对脂蛋白脂肪酶释放的公认影响一致，并表明肝素作用与组织摄取脂蛋白甘油三酯分解产物的能力之间存在直接关系。它们还证明了脂肪组织代谢来自脂蛋白甘油酯水解的甘油的能力。
