Lasunción M A, Herrera E
Arch Int Physiol Biochim. 1980 Dec;88(5):437-41. doi: 10.3109/13813458009092917.
It has been proposed that the lipolytic effect of serum is based on the presence of either lipoproteins or catecholamines. To test these hypotheses, pieces of epididymal fat pads from fed rats were incubated in the presence of albumin and glucose for 120 min. The addition of rat serum (5 mul/vial) enhanced the rates of both glycerol release to the media and [U-14C] glycerol utilization by the tissue. Heparin did not alter these parameters or the response produced by serum. VLDL from rat plasma also enhanced glycerol release and utilization for the formation of CO2 and lipids, and heparin significantly augmented these effects. Neither of the conditions studied affected the percentual distribution of 14C-lipid fractions in the tissues. It is known that in similar conditions to those used in the present study, adrenaline produces a decrease in the utilization of glycerol. Thus our findings do not support the proposed hypothesis explaining the fat-mobilizing action of serum, the mechanism of which remains to be determined.
有人提出，血清的脂解作用基于脂蛋白或儿茶酚胺的存在。为了验证这些假设，将喂食大鼠的附睾脂肪垫碎片在白蛋白和葡萄糖存在的情况下孵育120分钟。添加大鼠血清（5微升/管）提高了甘油释放到培养基中的速率以及组织对[U-14C]甘油的利用率。肝素没有改变这些参数或血清产生的反应。大鼠血浆中的极低密度脂蛋白（VLDL）也提高了甘油释放和用于形成二氧化碳和脂质的利用率，并且肝素显著增强了这些作用。所研究的任何条件都未影响组织中14C-脂质组分的百分比分布。众所周知，在与本研究中使用的类似条件下，肾上腺素会导致甘油利用率降低。因此，我们的研究结果不支持所提出的解释血清脂肪动员作用的假设，其机制仍有待确定。
