Koch F, Mehlhorn G, Neumeister K, Johannsen U, Panndorf H
Arch Exp Veterinarmed. 1980;34(6):851-9.
Five experiment series were applied to 38 calves, aged between two-and-a-half and four months, using whole-body exposure to X-ray treatment based on median lethal doses (MLD) between 1.2 Gy and 1.7 Gy. Various methods of immunisation were simultaneously applied, and their effects on irradiation-caused response of gamma globulin concentrations in blood serum were studied. Gamma globulin levels were determined by Kunkel's zinc sulphate turbidity test. Immunisation applied between 14 and 21 days prior to irradiation resulted on obvious stimulation of gamma globulin production. In two experiments with parenteral antigen application, gamma globulin levels increased through about 14 days in apparently irradiated animals declined, after that period of time, but they continued to rise in really irradiated probands. A high lethal dose of 1.7 Gy, however, was followed by significant drop, three weeks after irradiation, that is in concomitance with the climax of the radiation syndrome. When 1.5 Gy were used, rise in gamma globulin concentration was recordable also following oral administration of antigen. Response of irradiated animals was even more clearly pronounced by secondary reaction of antibody formation, following booster action due to experimental infection with homologous germs. The stimulating effect of irradiation upon gamma globulin levels in blood serum, when oral or parenteral immunisation had taken place, prior to irradiation, has been attributed to reaction of the immune-globulin producing system to the release of tissue proteins or tissue antigens. Immunisation, prior to irradiation, stimulated the immunological system which underwent stronger regeneration, after irradiation, or had obviously acquired higher resistance to radiation.
对38头年龄在两个半月至四个月之间的小牛进行了五个实验系列，采用基于1.2戈瑞至1.7戈瑞之间的半数致死剂量（MLD）的全身X射线照射治疗。同时应用了各种免疫方法，并研究了它们对辐射引起的血清中γ球蛋白浓度反应的影响。γ球蛋白水平通过昆克尔硫酸锌浊度试验测定。在照射前14至21天进行免疫导致γ球蛋白产生明显受到刺激。在两个采用注射抗原的实验中，在明显受照射的动物中γ球蛋白水平在约14天内升高，之后下降，但在真正受照射的受试动物中它们继续上升。然而，1.7戈瑞的高致死剂量在照射后三周导致显著下降，这与放射综合征的高峰期同时出现。当使用1.5戈瑞时，口服抗原后γ球蛋白浓度也有升高记录。由于同源细菌的实验性感染进行加强免疫后，抗体形成的二次反应使受照射动物的反应更加明显。当在照射前进行口服或注射免疫时，照射对血清中γ球蛋白水平的刺激作用归因于免疫球蛋白产生系统对组织蛋白或组织抗原释放的反应。照射前的免疫刺激了免疫系统，该系统在照射后经历更强的再生，或明显获得了更高的抗辐射能力。
