大鼠肝脏DNA聚合酶β：RNA依赖性和DNA依赖性活性的热失活

Rat liver DNA polymerase-beta: thermal inactivation of RNA- and DNA-dependent activities.

作者信息

Rossignol J M, Tillit J, de Recondo A M

出版信息

Biochimie. 1981 Jan;63(1):31-6. doi: 10.1016/s0300-9084(81)80143-5.

Abstract

Purified DNA polymerase-beta from rat liver was exposed to thermal inactivation and the remaining activities were then measured either with a hybrid template such as poly(A).(dT)12-18 (R-activity) or with a DNA template such as poly(dA).(dT)12-18 (D-activity). Time course of inhibition of R- and D-activities were identical. Neither activity was protected when the thermal treatment was performed in the presence of the template or dNTPs.

摘要

将从大鼠肝脏中纯化得到的DNA聚合酶β进行热失活处理，然后分别用诸如聚（A）·（dT）12-18的杂交模板（R活性）或诸如聚（dA）·（dT）12-18的DNA模板（D活性）来测量剩余活性。R活性和D活性的抑制时间进程是相同的。当在模板或脱氧核苷三磷酸存在的情况下进行热处理时，两种活性均未得到保护。