Simpson M S
Calcif Tissue Int. 1981;33(2):101-4. doi: 10.1007/BF02409420.
In view of the belief that tetracycline is removed from bone when it is demineralized, it was decided to investigate the effect of demineralizing agents on human dentine. Teeth which had been stained in vivo with tetracycline were fixed in formalin and demineralized using mineral and organic acids and EDTA at neutral pH. When examined microscopically under ultraviolet light, the tetracycline lines in dentine, although reduced in intensity, were all still present. This indicates that formalin fixation does not appreciably alter the fluorescent effect of tetracycline in teeth and that it is not removed by demineralization. It seems likely therefore that tetracycline is incorporated in both the mineral and organic phases of human dentine.
鉴于人们认为四环素在骨脱矿时会从骨中去除，因此决定研究脱钙剂对人牙本质的影响。体内用四环素染色的牙齿用福尔马林固定，并用无机酸、有机酸和中性pH值的乙二胺四乙酸（EDTA）进行脱矿。在紫外光下进行显微镜检查时，牙本质中的四环素带尽管强度降低，但仍然全部存在。这表明福尔马林固定不会明显改变四环素在牙齿中的荧光效应，并且脱矿不会将其去除。因此，四环素似乎同时掺入了人牙本质的无机相和有机相中。
