Colcher D, Horan Hand P, Teramoto Y A, Wunderlich D, Schlom J
Cancer Res. 1981 Apr;41(4):1451-9.
Monoclonal antibodies have been generated against disrupted mammary tumor viruses isolated from Mus musculus, Mus cervicolor, and Mus cookii. Monoclonal antibodies directed against the M.W. 36,000 external glycoproteins of these viruses demonstrate the presence of multiple epitopes, i.e., distinct antigenic determinants, within these glycoproteins. These epitopes represent type, group, and interspecies determinants. Monoclonal antibodies have also been used to define multiple epitopes on the M.W. 28,000 major internal polypeptides of murine mammary tumor viruses that exhibit both type- and group-specific determinants. The monoclonal antibodies to the M.W. 36,000 glycoprotein and the M.W. 28,000 polypeptide have been used to distinguish all six mammary tumor virus isolates of M. musculus from each other, including both endogenous and exogenous viruses from the same strain, and a new virus isolate from BALB/c mice. With the use of the immunoperoxidase technique, the monoclonal antibodies generated have been used to demonstrate a heterogeneity of expression of mammary tumor virus gene products in primary mammary tumors of three Mus species. These studies have revealed that a given antigenic determinant may be expressed differentially in mammary tumors of two different Mus species, among mammary tumors of the same Mus species, and, at times, in different areas of the same mammary tumor.
已经制备出了针对从小鼠、姬鼠和库克氏鼠中分离出的破碎乳腺肿瘤病毒的单克隆抗体。针对这些病毒分子量为36,000的外部糖蛋白的单克隆抗体表明，这些糖蛋白中存在多个表位，即不同的抗原决定簇。这些表位代表型、组和种间决定簇。单克隆抗体也已用于确定小鼠乳腺肿瘤病毒分子量为28,000的主要内部多肽上的多个表位，这些多肽表现出型特异性和组特异性决定簇。针对分子量为36,000的糖蛋白和分子量为28,000的多肽的单克隆抗体已被用于区分小家鼠的所有六种乳腺肿瘤病毒分离株，包括来自同一品系的内源性和外源性病毒，以及来自BALB/c小鼠的一种新病毒分离株。利用免疫过氧化物酶技术，所产生的单克隆抗体已被用于证明三种鼠类原发性乳腺肿瘤中乳腺肿瘤病毒基因产物表达的异质性。这些研究表明，给定的抗原决定簇在两种不同鼠类的乳腺肿瘤中、在同一鼠类的乳腺肿瘤之间，有时在同一乳腺肿瘤的不同区域可能会有不同的表达。
