Horiuchi K, Fujimoto D, Fukushima M, Kanai K
Cancer Res. 1981 Apr;41(4):1488-91.
Rates of histone acetylation and deacetylation in nuclei from fetal, adult, and two kinds of neoplastic rat hepatocytes were examined. Histone acetylation in isolated nuclei was measured in the presence of 6 mM sodium n-butyrate, a potent inhibitor of deacetylase, and in the absence of the inhibitor. The deacetylase activity was estimated from the difference between the rates with or without the inhibitor. Both histone acetylation and deacetylation in nuclei from hepatoma cells (AH 66 cells) occurred two times faster than those of nuclei from fetal and adult livers regardless of alpha-fetoprotein production. This increased acetylation and deacetylation in hepatoma cells may be ascribed to either the increased activities of the enzymes or the increased accessibility of histone to the enzymes in the chromatin. Autoradiographic analysis of acetylated histones showed that all of the internal histones of the nucleosomes were acetylated and that apparent difference was found in the pattern of acetylated fractions between hepatoma nuclei and normal liver cell nuclei.
检测了来自胎儿、成年大鼠以及两种肿瘤性大鼠肝细胞的细胞核中组蛋白乙酰化和去乙酰化的速率。在存在6 mM丁酸钠（一种有效的去乙酰化酶抑制剂）和不存在该抑制剂的情况下，测定分离细胞核中的组蛋白乙酰化水平。通过有或无抑制剂时速率的差异来估算去乙酰化酶活性。无论甲胎蛋白的产生情况如何，肝癌细胞（AH 66细胞）细胞核中的组蛋白乙酰化和去乙酰化速度都比胎儿和成年肝脏细胞核中的快两倍。肝癌细胞中这种乙酰化和去乙酰化的增加可能归因于酶活性的增加或染色质中组蛋白对酶的可及性增加。对乙酰化组蛋白的放射自显影分析表明，核小体的所有内部组蛋白都被乙酰化，并且在肝癌细胞核和正常肝细胞细胞核之间的乙酰化组分模式中发现了明显差异。
