Horiuchi K, Fujimoto D, Uto N
J Exp Zool. 1984 Jul;231(1):75-80. doi: 10.1002/jez.1402310110.
Nuclei were isolated from sea urchin (Hemicentrotus pulcherrimus) embryos during early stages of development, and the rates of histone acetylation and deacetylation were examined. Histone acetylation was measured in the presence of 6 mM sodium n-butyrate, a potent inhibitor of deacetylase, and in the absence of the inhibitor. The deacetylation activity was estimated from the difference between the rates with and without the inhibitor. The activities of the acetylation and the deacetylation, and the ratio of deacetylation to acetylation were unchanged during the stages between early blastula and gastrula. Autoradiographic analysis of acetylated histones showed that all of the internal histones of the nucleosomes were acetylated and that no apparent difference was found in the pattern of the acetylated fractions during the stages compared.
在发育早期阶段从海胆（光棘球海胆）胚胎中分离出细胞核，并检测组蛋白乙酰化和去乙酰化的速率。在存在6 mM丁酸钠（一种有效的脱乙酰酶抑制剂）和不存在该抑制剂的情况下测量组蛋白乙酰化。通过有抑制剂和无抑制剂时速率的差异来估计去乙酰化活性。在囊胚早期到原肠胚阶段之间，乙酰化和去乙酰化活性以及去乙酰化与乙酰化的比率没有变化。对乙酰化组蛋白的放射自显影分析表明，核小体的所有内部组蛋白都被乙酰化，并且在比较的阶段中乙酰化组分的模式没有明显差异。
