Valet J P, Marceau N, Deschênes J
Cell Biol Int Rep. 1981 Mar;5(3):307-14. doi: 10.1016/0309-1651(81)90229-0.
Hepatocytes were isolated from 1-day and 1,2,3 and 12-week old rat livers by collagenase perfusion and the relative numbers of albumin (ALB) and alpha-fetoprotein (AFP) producers were evaluated using the reverse hemolytic plaque assay. The percentage of ALB producers remained essentially constant to 35% over the 12-week period. In contrast, the percentage of AFP producers varied from 19% at 1 day up to 28% at 1 week and then down to 0.1% at 3 weeks. Moreover, a double identification of secreting hepatocytes, using an adaptation of the plaque assay, demonstrated that AFP producing hepatocytes were also ALB producers. These results are explained in terms of a restricted specialization of differentiating hepatocytes during normal development.
通过胶原酶灌注从1日龄、1周龄、2周龄、3周龄和12周龄大鼠的肝脏中分离出肝细胞，并使用反向溶血空斑试验评估白蛋白（ALB）和甲胎蛋白（AFP）产生细胞的相对数量。在12周的时间里，产生ALB的细胞百分比基本保持在35%不变。相比之下，产生AFP的细胞百分比从1日龄时的19%变化到1周龄时的28%，然后在3周龄时降至0.1%。此外，通过对空斑试验进行改进，对分泌肝细胞进行双重鉴定，结果表明产生AFP的肝细胞也是产生ALB的细胞。这些结果可以用正常发育过程中分化肝细胞的有限特化来解释。
