Ito Y, Aoki H, Kimura Y, Takano M, Maeno K, Shimokata K
Infect Immun. 1980 Aug;29(2):383-9. doi: 10.1128/iai.29.2.383-389.1980.
When spleen cells derived from C57BL/6 mice immunized with L cells 7 days previously were cocultured with antigenic cells, immune interferon appeared in the culture fluid. We analyzed the tissue distribution of the immune interferon-producing cells (IIPC) which appeared in various lymphoid organs after allogeneic stimulation. Although fluid from cocultures of L-cell-sensitized thymocytes and L-cells could not detect interferon activity consistently, small numbers of IIPC could be detected by using the enumeration method of IIPC. The generation, maintenance, and nature of IIPC emerging in the spleen were different depending on how the host mice were immunized. Multiple antigenic stimulations were more effective and induced longer-lasting immune interferon production than a single stimulation. IIPC induced by a single stimulation appeared to be sensitive to cortisone, vinblastine, and cyclophosphamide and were relatively short lived. In contrast, IIPC induced by multiple stimulations seemed to be partially resistant to these drugs and long lived. When mice were immunized with intact L-cells, carrageenan, a known antimacrophage agent, had no effect on immune interferon production. However, when mice were immunized with solubilized L-cell antigen, this drug displayed a suppressive effect on immune interferon production.
当用7天前免疫过L细胞的C57BL/6小鼠的脾细胞与抗原细胞共培养时，培养液中出现了免疫干扰素。我们分析了同种异体刺激后出现在各种淋巴器官中的免疫干扰素产生细胞（IIPC）的组织分布。虽然L细胞致敏的胸腺细胞与L细胞共培养的培养液不能持续检测到干扰素活性，但使用IIPC计数方法可检测到少量的IIPC。根据宿主小鼠的免疫方式不同，脾中出现的IIPC的产生、维持和性质也有所不同。多次抗原刺激比单次刺激更有效，能诱导产生更持久的免疫干扰素。单次刺激诱导的IIPC似乎对可的松、长春碱和环磷酰胺敏感，存活时间相对较短。相比之下，多次刺激诱导的IIPC似乎对这些药物有部分抗性，存活时间较长。当用完整的L细胞免疫小鼠时，已知的抗巨噬细胞剂角叉菜胶对免疫干扰素的产生没有影响。然而，当用溶解的L细胞抗原免疫小鼠时，这种药物对免疫干扰素的产生有抑制作用。
