Lett-Brown M A, Thueson D O, Grant J A
Int Arch Allergy Appl Immunol. 1981;64(3):241-8. doi: 10.1159/000232700.
Guinea pig blood was found to contain an average of 106 +/- 21 ng/ml of histamine. Of this total, approximately 85-90% was of platelet origin and the rest from basophils. Basophils contain about 0.72 pg of histamine/cell. Concanavalin A (1-5 microgram/ml) induced the release of approximately 65% of the basophilic histamine. When basophils were isolated from animals sensitized to ovalbumin or keyhole limpet hemocyanin, addition of the appropriate antigen induced histamine release at concentrations of 0.01 microgram/ml or lower. Individual animals were studied over time by repetitive bleeding. The circulating basophils remained sensitized for at least 17 weeks postsensitization. However, release did not occur if animals had been sensitized less than 7 days earlier. This assay facilitates the investigation of basophil sensitization since animals can be studied on several occasions following immunization. The mechanisms, timing and role of basophil sensitization in various types of immune and hypersensitivity reactions can now be evaluated.
发现豚鼠血液中组胺的平均含量为106±21纳克/毫升。在这一总量中，约85 - 90%源自血小板，其余来自嗜碱性粒细胞。嗜碱性粒细胞每个细胞含约0.72皮克组胺。伴刀豆球蛋白A（1 - 5微克/毫升）可诱导约65%的嗜碱性组胺释放。当从对卵清蛋白或钥孔戚血蓝蛋白致敏的动物中分离出嗜碱性粒细胞时，加入适当抗原在浓度为0.01微克/毫升或更低时即可诱导组胺释放。通过重复采血对个体动物进行长期研究。循环嗜碱性粒细胞在致敏后至少17周仍保持致敏状态。然而，如果动物在7天内致敏则不会发生释放。该检测方法有助于研究嗜碱性粒细胞致敏情况，因为动物在免疫后可多次进行研究。现在可以评估嗜碱性粒细胞致敏在各种免疫和超敏反应中的机制、时间及作用。
