Undem B J, Brendel J B, Hirth T, Buckner C K, Graziano F M
Am Rev Respir Dis. 1986 May;133(5):763-8.
Guinea pig lung mast cells and blood basophils were isolated and purified and their mediator release characteristics were compared. Upon stimulation with the antigen ovalbumin (OA) of cells passively sensitized with antiovalbumin (anti-OA) antibody, both cell types released histamine. The sensitivity and maximal response (20 to 25% histamine release) to OA was similar for both cells and was unaffected by cell purification. Antigen-induced histamine release (HR) was dependent upon added calcium to a similar extent (1 mM Ca++ maximal release) in both cell types; OA stimulation of passively sensitized mast cells also released leukotriene bioactivity (maximal release, 52 +/- 7 units/10(6) mast cells). There was no correlation between OA-induced leukotriene release and mast cell purity. No leukotriene bioactivity was detected in actively (sheep blood sensitization) or passively (anti-OA) sensitized basophils. Both lung mast cells and blood basophils released histamine in response to the secretagogues calcium ionophore A23187 and 12-O-tetradecanoylphorbol-13-acetate (TPA); TPA-induced HR from mast cells was independent of added calcium. In basophils, TPA-induced HR was only partially independent of added calcium. While both cell types were poorly responsive to the secretagogue 48/80, only the lung mast cell demonstrated inconsistent HR to concanavalin A (Con-A). Phosphatidylserine had no effect on HR provoked by antigen, Con-A, or compound 48/80. These observations demonstrate similarities and differences in mediator release characteristics between guinea pig lung mast cells and blood basophils that are similar to those observed with human lung mast cells and basophils. These observations also suggest a lack of influence on mediator release by other cell types present in dispersed lung cell and mixed leukocyte preparations.
分离并纯化豚鼠肺肥大细胞和血液嗜碱性粒细胞，比较它们的介质释放特性。用抗卵清蛋白（抗 - OA）抗体被动致敏的细胞，在受到卵清蛋白（OA）抗原刺激时，两种细胞类型均释放组胺。两种细胞对OA的敏感性和最大反应（组胺释放20%至25%）相似，且不受细胞纯化的影响。抗原诱导的组胺释放（HR）在两种细胞类型中都在相似程度上依赖于添加的钙（1 mM Ca++时最大释放）；OA刺激被动致敏的肥大细胞也释放白三烯生物活性（最大释放量为52±7单位/10(6)个肥大细胞）。OA诱导的白三烯释放与肥大细胞纯度之间没有相关性。在主动（绵羊血致敏）或被动（抗 - OA）致敏的嗜碱性粒细胞中未检测到白三烯生物活性。肺肥大细胞和血液嗜碱性粒细胞均对促分泌剂钙离子载体A23187和12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）产生组胺释放反应；TPA诱导的肥大细胞组胺释放不依赖于添加的钙。在嗜碱性粒细胞中，TPA诱导的组胺释放仅部分不依赖于添加的钙。虽然两种细胞类型对促分泌剂48/80反应不佳，但只有肺肥大细胞对伴刀豆球蛋白A（Con - A）表现出不一致的组胺释放反应。磷脂酰丝氨酸对抗原、Con - A或化合物48/80引发的组胺释放没有影响。这些观察结果表明豚鼠肺肥大细胞和血液嗜碱性粒细胞在介质释放特性方面的异同，与在人肺肥大细胞和嗜碱性粒细胞中观察到的相似。这些观察结果还表明，分散的肺细胞和混合白细胞制剂中存在的其他细胞类型对介质释放没有影响。
