Tanaka Y, Sugamura K, Hinuma Y
J Immunol. 1982 Mar;128(3):1241-5.
Clones of human cytotoxic T cells (Tc) specific for Epstein Barr virus (EBV) were isolated from peripheral blood lymphocyte (PBL) cultures stimulated repeatedly with autologous EBV-transformed lymphoblastoid cell line (LCL) cells in vitro. The method employed to clone EBV-specific Tc was a limiting dilution technique utilizing T cell growth factor (TCGF). The EBV specificity of Tc clones was determined by showing that they were significantly cytotoxic for autologous LCL cells but not for either autologous PBL or (natural killer-sensitive) K-562 cells. Eight EBV-specific Tc clones derived from a single donor exhibited distinct cytotoxic patterns against allogeneic LCL targets. Two clones were cytotoxic to LCL targets sharing both HLA-A26 and B15 antigens with effectors, and killing by two other clones was strongly restricted to autologous LCL cells. The four remaining clones showed cytotoxicities against various allogeneic LCL targets irrespective of HLA antigen expression. Eight EBV-specific Tc clones derived from a second donor also exhibited a wide spectrum of cytotoxicity to allogeneic LcL targets. We conclude that EBV-specific Tc, induced in vitro, consist of a number of clones with respect to restrictions imposed by the major histocompatibility complex. The determinants regulating these restrictions may include not only private HLA antigenic determinants that are defined by the HLA serotyping, but also undefined HLA antigenic determinants.
从体外经自体EB病毒（EBV）转化的淋巴母细胞系（LCL）细胞反复刺激的外周血淋巴细胞（PBL）培养物中分离出了对EBV特异的人细胞毒性T细胞（Tc）克隆。用于克隆EBV特异Tc的方法是利用T细胞生长因子（TCGF）的有限稀释技术。通过证明Tc克隆对自体LCL细胞有显著细胞毒性，但对自体PBL或（对自然杀伤敏感的）K - 562细胞无细胞毒性，来确定Tc克隆的EBV特异性。来自单个供体的8个EBV特异Tc克隆对同种异体LCL靶标表现出不同的细胞毒性模式。两个克隆对与效应细胞共享HLA - A26和B15抗原的LCL靶标具有细胞毒性，另外两个克隆的杀伤作用强烈局限于自体LCL细胞。其余4个克隆对各种同种异体LCL靶标均表现出细胞毒性，而不考虑HLA抗原表达情况。来自第二个供体的8个EBV特异Tc克隆对同种异体LcL靶标也表现出广泛的细胞毒性谱。我们得出结论，体外诱导的EBV特异Tc在主要组织相容性复合体施加的限制方面由多个克隆组成。调节这些限制的决定因素可能不仅包括由HLA血清分型定义的个体HLA抗原决定因素，还包括未定义的HLA抗原决定因素。
