生物组织中硬矿物纤维的切片与成像
Sectioning and imaging hard mineral fibres in biological tissues.
作者信息
Johnson N F, Ibe K
出版信息
J Microsc. 1981 Apr;122(Pt 1):87-92. doi: 10.1111/j.1365-2818.1981.tb01245.x.
Abstract
A technique is described which overcomes the problems associated with sectioning biological tissue containing hard mineral fibres. 0.2--0.5 micrometer thick sections were cut with a diamond knife, placed in a folding grid, conventionally stained with uranyl acetate and lead citrate and viewed at an accelerating voltage of 200 kV in the scanning transmission mode.
摘要
本文描述了一种技术，该技术克服了与含有硬矿物纤维的生物组织切片相关的问题。用金刚石刀切割出0.2 - 0.5微米厚的切片，将其置于折叠网格中，常规用醋酸铀和柠檬酸铅染色，然后在扫描透射模式下以200 kV的加速电压进行观察。
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