Sloane J P, Ormerod M G
Cancer. 1981 Apr 1;47(7):1786-95. doi: 10.1002/1097-0142(19810401)47:7<1786::aid-cncr2820470711>3.0.co;2-8.
An antiserum raised against human milk fat globule membranes has been used to stain a wide variety of human tissues by the indirect immunoperoxidase method. The antigen detected has been designated Epithelial Membrane Antigen and was confined to the luminal and surface membranes (and to a lesser extent the cytoplasm) of epithelial tissues in the normal state. Increased staining was observed in a variety of nonneoplastic disease states as well as in many neoplasms. The staining of tumors is related both to their histogenesis and degree of differentiation, being found only in lesions of surface epithelial or mesothelial origin and being more consistently present in well and moderately differentiated neoplasms. However, strong staining was also present in many anaplastic tumors and this together with the resistance of the antigen to formol fixation and paraffin embedding suggest that the antiserum has a valuable role to play in diagnostic tumor histopathology as an indicator of epithelial differentiation. The distinction of anaplastic carcinomas from malignant lymphomas and the recognition of spindle-cell epithelial malignancies are especially useful applications. The identification of minute metastatic deposits of carcinoma in organs such as the liver and bone marrow is also greatly facilitated. Malignant neoplasms often exhibit patterns of staining different from those of the normal tissues. The possible significance of these patterns is discussed.
一种针对人乳脂肪球膜产生的抗血清已被用于通过间接免疫过氧化物酶法对多种人体组织进行染色。所检测到的抗原被命名为上皮膜抗原，在正常状态下局限于上皮组织的腔面膜和表面膜（以及程度较轻的细胞质）。在多种非肿瘤性疾病状态以及许多肿瘤中均观察到染色增强。肿瘤的染色与其组织发生和分化程度相关，仅在表面上皮或间皮起源的病变中发现，并且在高分化和中分化肿瘤中更持续存在。然而，许多间变性肿瘤中也存在强染色，并且该抗原对福尔马林固定和石蜡包埋具有抗性，这表明该抗血清作为上皮分化的指标在诊断肿瘤组织病理学中具有重要作用。间变性癌与恶性淋巴瘤的鉴别以及梭形细胞上皮恶性肿瘤的识别是特别有用的应用。在肝脏和骨髓等器官中微小癌转移灶的识别也大大得到促进。恶性肿瘤通常表现出与正常组织不同的染色模式。讨论了这些模式可能的意义。
