Sloane J P, Hughes F, Ormerod M G
Histochem J. 1983 Jul;15(7):645-54. doi: 10.1007/BF01002986.
Using standard immunohistological techniques on formalin-fixed paraffin-embedded sections, we have evaluated the role of epithelial membrane antigen in the histopathological diagnosis of tumours. Of the 70 samples examined, 22 were taken for staging purposes from patients known to have carcinoma and, in half these cases, malignant cells were seen which could not be identified with confidence by conventional means. Forty-eight tumours were stained in order to determine their histogenesis. Twenty-two of these were positive and 20 subsequently proved to be epithelial on follow-up studies, including six in which a diagnosis of non-epithelial malignancy had been made on conventional preparations. The distinction of anaplastic carcinoma from malignant lymphoma and of spindle-cell carcinoma from sarcoma were the most useful applications. One of the positive tumours was of germ cell origin and in one the histogenesis is still not clear. Comparison with carcinoembryonic antigen and pre-keratin showed that epithelial membrane antigen was the most sensitive marker of epithelial differentiation in formalin-fixed tissue. A combination of all three reagents, though, increases diagnostic accuracy and allows tentative suggestions to be made about the possible site of origin of a metastatic carcinoma.
我们运用标准免疫组织学技术对福尔马林固定石蜡包埋切片进行研究，评估了上皮膜抗原在肿瘤组织病理学诊断中的作用。在检查的70个样本中，22个取自已知患有癌症的患者用于分期，在其中半数病例中，发现了常规方法无法确切识别的恶性细胞。对48个肿瘤进行染色以确定其组织发生。其中22个呈阳性，后续研究证实20个为上皮性肿瘤，包括6个在常规制片时被诊断为非上皮性恶性肿瘤的病例。鉴别间变性癌与恶性淋巴瘤以及梭形细胞癌与肉瘤是最有用的应用。其中一个阳性肿瘤起源于生殖细胞，还有一个的组织发生仍不清楚。与癌胚抗原和前角蛋白比较显示，上皮膜抗原是福尔马林固定组织中上皮分化最敏感的标志物。不过，联合使用这三种试剂可提高诊断准确性，并能对转移性癌的可能起源部位做出初步推断。
