Inhibitor of peritoneal mononuclear phagocyte colony forming cells.

We investigated an inhibitory factor present in mouse L-cell conditioned medium. This soluble factor, colony inhibitory factor (CIF), selectively inhibits the clonal growth of mouse peritoneal exudate (PE) macrophages in vitro. CIF appears to be a non-dialyzable macromolecule which was eluted by Sephadex G200 gel filtration at post-albumin fractions. It can be fractionated by ammonium sulfate precipitation within the range of 60%--70% saturated solution at room temperature. CIF is moderately heat labile and is destroyed by incubation at high pH. CIF is more effective on PE cells from C57 mice than on those from C3H or DBA mice. It also inhibits the clonal growth of committed stem cells for both granulocytes and macrophages present in bone marrow but to a much lesser degree. CIF is not interferon since no antiviral activity is demonstrable in the CIF preparation.