Lin H S, Kuhn C, Stewart C C
J Cell Physiol. 1978 Jul;96(1):133-8. doi: 10.1002/jcp.1040960116.
We investigated the effects of age, sex and strain in the induction of peritoneal exudate colony-forming cells (PE-CFC) in mice. Sex and age (ranging from 3 weeks to 12 months) had no significant effect on the induction of PE-CFC. However, we found significant difference between strains in terms of the number of nucleated cells and the proportion of PE-CFC in exudate cells. When we investigated the mechanisms behind this strain difference, we found that it was due to neither the type of colony-stimulating factor employed in culture, nor the type of stimulants used to induce the exudate, nor the difference in the kinetics of appearance of PE-CFC in the peritoneal cavity. We also studied the induction of PE-CFC in rats and hamsters and the growth of these cells in vitro. Unlike mouse cells, PE-CFC from rats and hamsters could also use media conditioned by cells from other species as a source of colony-stimulating factor.
我们研究了年龄、性别和品系对小鼠腹腔渗出液集落形成细胞（PE-CFC）诱导的影响。性别和年龄（3周龄至12月龄）对PE-CFC的诱导没有显著影响。然而，我们发现品系之间在有核细胞数量和渗出细胞中PE-CFC比例方面存在显著差异。当我们研究这种品系差异背后的机制时，我们发现这既不是由于培养中使用的集落刺激因子类型，也不是由于用于诱导渗出液的刺激剂类型，也不是由于腹腔中PE-CFC出现动力学的差异。我们还研究了大鼠和仓鼠中PE-CFC的诱导以及这些细胞在体外的生长。与小鼠细胞不同，大鼠和仓鼠的PE-CFC也可以使用其他物种细胞条件化的培养基作为集落刺激因子的来源。
