Sakakibara K, Tsukada Y
Gan. 1980 Oct;71(5):679-85.
The expression of two markers of fetal liver, alpha-fetoprotein (AFP) and gamma-glutamyltranspeptidase (GGT), and the relationship between these markers and tumorigenicity were studied in 17 continuous epithelial-like cell lines derived from normal rat livers and ascitic hepatoma cells. Fourteen cell lines positive for GGT activity included 6 non-transplantable lines. Out of 10 cell lines transplantable to syngeneic rats and/or immuno-depressed hamsters, 2 showed no activity of GGT cytochemically. AFP production was seen in the culture fluid of 4 out of 15 cell lines tested. They consisted of 1 GGT-positive, transplantable, 1 GGT-negative, transplantable, and 2 GGT-positive, non-transplantable cell lines. These results indicate that no correlation exists among these 3 parameters related to neoplastic and preneoplastic states of cells.
在从正常大鼠肝脏和腹水肝癌细胞衍生的17个连续上皮样细胞系中，研究了两种胎儿肝脏标志物甲胎蛋白（AFP）和γ-谷氨酰转肽酶（GGT）的表达，以及这些标志物与致瘤性之间的关系。14个GGT活性阳性的细胞系包括6个不可移植的细胞系。在可移植到同基因大鼠和/或免疫抑制仓鼠的10个细胞系中，有2个在细胞化学上未显示GGT活性。在所测试的15个细胞系中，有4个细胞系的培养液中可检测到AFP产生。它们包括1个GGT阳性、可移植的细胞系，1个GGT阴性、可移植的细胞系，以及2个GGT阳性、不可移植的细胞系。这些结果表明，与细胞的肿瘤性和肿瘤前状态相关的这3个参数之间不存在相关性。
