5-氮杂胞苷对哺乳动物富含G-C的异染色质的检测
Detection of G-C rich heterochromatin by 5-azacytidine in mammals.
作者信息
Viegas-Péquignot E, Dutrillaux B
出版信息
Hum Genet. 1981;57(2):134-7. doi: 10.1007/BF00282008.
PMID:6164628
Abstract
A new effect of 5-ACR (5-azacytidine), observed in fibroblast or kidney cell cultures is described in the calf (Bos taurus) and in some selected primates (Cebus capucinus, Erythrocebus patas, and Cercopithecus cephus). This cytidine analogue, used at low doses during the last late S phase, prevents the condensation of R-band positive heterochromatin (G-C rich segments). Thus, 5-ACR treatment can be used as a simple method for the detection of G-C rich heterochromatin.
摘要
在小牛（Bos taurus）和一些选定的灵长类动物（卷尾猴、赤猴和白顶白眉猴）中描述了在成纤维细胞或肾细胞培养物中观察到的5-氮杂胞苷（5-ACR）的一种新效应。这种胞苷类似物在最后一个S期后期以低剂量使用时，可防止R带阳性异染色质（富含G-C的片段）凝聚。因此，5-ACR处理可作为检测富含G-C的异染色质的一种简单方法。
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