de Bruijn W C, van Buitenen J M
Histochem J. 1981 Jan;13(1):125-36. doi: 10.1007/BF01005845.
Fibrin-enrobed, commercially produced glycogen was treated, without prior glutaraldehyde fixation, to a form of post-fixation with solutions of OsVIIIO4 or with a mixture of either OsVIIIO4 plus K3FeIII(CN)6 or K2OsVIO4 plus K4FeII(CN)6. Only the last mixture gave contrast staining of the glycogen in unstained ultrathin sections. The first mixture rendered the glycogen just barely visible but the glycogen contrast was increased by lead staining. The glycogen treated with the OsVIIIO4 solution was not contrast stained an was just observable after lead staining. Qualitative X-ray microanalysis of the glycogen in the ultrathin sections confirmed the presence of osmium and iron in the glycogen treated with both mixtures. The glycogen treated with OsVIIIO4 alone was difficult to analyse. Quantitative X-ray microanalysis showed that, in the glycogen treated with the OsVIIIO4 mixture plus K3FeIII(CN)6, the mean atomic osmium to iron ratio was 1:5. In the glycogen treated with K2OsVIO4 plus K4FeII(CN)6, this ratio was 1:17. However, th mean net osmium intensity in the latter case was 15 times higher than in the former case and for the iron even 40 times higher.
用商业生产的包裹有纤维蛋白的糖原，在未预先用戊二醛固定的情况下，用OsVIIIO4溶液或OsVIIIO4与K3FeIII(CN)6的混合物或K2OsVIO4与K4FeII(CN)6的混合物进行后固定处理。只有最后一种混合物能使未染色的超薄切片中的糖原产生反差染色。第一种混合物使糖原勉强可见，但通过铅染色可增加糖原的反差。用OsVIIIO4溶液处理的糖原未产生反差染色，仅在铅染色后才可观察到。对超薄切片中糖原的定性X射线微分析证实，用两种混合物处理的糖原中均存在锇和铁。单独用OsVIIIO4处理的糖原难以分析。定量X射线微分析表明，在用OsVIIIO4混合物加K3FeIII(CN)6处理的糖原中，平均原子锇与铁的比例为1:5。在用K2OsVIO4加K4FeII(CN)6处理的糖原中，该比例为1:17。然而，后一种情况下的平均净锇强度比前一种情况高15倍，铁的强度甚至高40倍。
