de Bruijn W C, van Buitenen J M
J Histochem Cytochem. 1980 Nov;28(11):1242-50. doi: 10.1177/28.11.6159393.
The composition of the contrast-donating complex of rat liver glycogen, nucleoplasm, erythrocytes, and mitochondria was established by X-ray microanalysis. In these compartments the presence of osmium and iron was shown qualitatively in tissue after glutaraldehyde fixation, treated with OsVIIIO4 plus K4FeII(CN)6 and in similar tissue treated with a combination of K2OsVIO4 plus K4FeII(CN)6. Osmium and ruthenium were detected in these compartments, in aldehyde-fixed tissue treated with mixtures containing K2RuIVL(CN)6 rather than K4FeII(CN)6. The iron detected in the glycogen, nucleoplasm, erythrocytes, and mitochondria of tissue treated with K2RuIV(CN)6 mixtures proved to derive from sources inside the electron microscope, and had to be considered an artifact. Quantitatively, the mean atomic ratios of osmium-to-iron and osmium-to-ruthenium were determined from spectra obtained by point analyses of the same compartments (glycogen, nucleoplasm, mitochondria, lipid droplets, and erythrocytes). After correction of the spectra for the instrumental iron contribution, the osmium-to-iron and osmium-to-ruthenium ratios in the glycogen were about 1:3 for tissue treated with those combinations including K2OsVIO4. In the other compartments, the osmium-to-iron and osmium-to-ruthenium ratios were virtually 1:0. For Os-VIIIO4 in combination with potassium ferrouscyanide however the osmium-to-iron ratio was 1:7 in the glycogen and 1:5 in all other compartments. OsVIIIO4 was combined with potassium ruthenium-cyanide, the osmium-to-ruthenium ratio was 1:2 in the glycogen and 2:1 in the other compartments. These results support our view that the selective glycogen contrast is obtained by complex formation.
通过X射线微量分析确定了大鼠肝脏糖原、核质、红细胞和线粒体中造影剂供体复合物的组成。在这些区室中，经戊二醛固定后的组织在用OsVIIIO4加K4FeII(CN)6处理后，以及在用K2OsVIO4加K4FeII(CN)6组合处理的类似组织中，定性显示了锇和铁的存在。在用含有K2RuIVL(CN)6而非K4FeII(CN)6的混合物处理的醛固定组织中，在这些区室中检测到了锇和钌。在用K2RuIV(CN)6混合物处理的组织的糖原、核质、红细胞和线粒体中检测到的铁被证明来自电子显微镜内部的来源，必须被视为一种假象。定量地，通过对相同区室（糖原、核质、线粒体、脂滴和红细胞）进行点分析获得的光谱确定了锇与铁和锇与钌的平均原子比。在对光谱进行仪器铁贡献校正后，对于用包括K2OsVIO4的那些组合处理的组织，糖原中锇与铁和锇与钌的比率约为1:3。在其他区室中，锇与铁和锇与钌的比率实际上为1:0。然而，对于Os-VIIIO4与亚铁氰化钾的组合，糖原中锇与铁的比率为1:7，在所有其他区室中为1:5。当OsVIIIO4与钌氰化钾结合时，糖原中锇与钌的比率为1:2，在其他区室中为2:1。这些结果支持了我们的观点，即通过复合物形成获得选择性糖原造影。
