大鼠腹膜肥大细胞脱颗粒过程中糖缀合物的细胞化学定位
Cytochemical localization of glycoconjugates in rat peritoneal mast cells during degranulation.
作者信息
Poon K C, Sannes P L, Simson J A, Spicer S S
出版信息
Histochem J. 1981 Jan;13(1):23-30. doi: 10.1007/BF01005836.
PMID:6164671
Abstract
Cytochemical methods for the localization of glycoconjugates including concanavalin A-horseradish peroxidase (ConA-HRP) and dialysed iron were used to study the distribution of glycoconjugates in mast cell granules during degranulation. The ConA-HRP method revealed intense staining of discharged mast cell granules. Dialysed iron staining was seen at the granule periphery, with extruded granules exhibiting more intense staining than undischarged granules.
摘要
采用包括伴刀豆球蛋白A-辣根过氧化物酶（ConA-HRP）和透析铁在内的糖缀合物定位细胞化学方法，研究脱颗粒过程中肥大细胞颗粒中糖缀合物的分布。ConA-HRP方法显示已排出的肥大细胞颗粒有强烈染色。透析铁染色见于颗粒周边，排出的颗粒比未排出的颗粒染色更强。
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