Wetzel M G, Wetzel B K, Spicer S S
J Cell Biol. 1966 Aug;30(2):299-315. doi: 10.1083/jcb.30.2.299.
Selective ultrastructural staining of acid mucosubstances in sites containing histochemically identifiable sulfo- and sialomucins has been obtained in fixed cryostat sections with both ferric chloride and colloidal iron solutions. The rectosigmoid region of mouse colon was fixed in glutaraldehyde, formalin, or phosphate-buffered osmium tetroxide, and 40 micro cryostat sections of this material were treated with 0.1 to 0.4% ferric chloride or with a solution of dialyzed ferric chloride, ammonia, and glycerin. Specific staining depended upon the pH of the iron-containing solutions, and the optimal value was found to be approximately 2.0. Specific localization of acid mucosubstances has been noted in intracellular sites, including globules within colonic goblet cells and "deep crypt" mucous cells, small vesicles of the superficial nongoblet epithelial cells, and Golgi lamellae within each of these cell types. Extracellular material, presumed to be acid mucosubstance, was found on the surface of the epithelial microvilli and on the lumenal surface of capillary endothelium. Similar material formed a reticular network surrounding stromal cells, collagen bundles, and various colonic connective tissue elements.
在含有组织化学可识别的硫酸粘蛋白和唾液酸粘蛋白的部位，用氯化铁溶液和胶体铁溶液对固定的低温恒温器切片中的酸性粘多糖进行选择性超微结构染色已获成功。小鼠结肠的直肠乙状结肠区域用戊二醛、福尔马林或磷酸盐缓冲四氧化锇固定，对该材料的40微米低温恒温器切片用0.1%至0.4%的氯化铁溶液或用透析过的氯化铁、氨和甘油溶液处理。特异性染色取决于含铁溶液的pH值，发现最佳值约为2.0。已注意到酸性粘多糖在细胞内部位的特异性定位，包括结肠杯状细胞内的小球和“深部隐窝”粘液细胞、浅表非杯状上皮细胞的小泡以及这些细胞类型中每一种的高尔基体薄片。推测为酸性粘多糖的细胞外物质存在于上皮微绒毛表面和毛细血管内皮的管腔表面。类似的物质形成围绕基质细胞、胶原束和各种结肠结缔组织成分的网状网络。
