Bass D A, Lewis J C, Szejda P, Cowley L, McCall C E
Lab Invest. 1981 May;44(5):403-9.
Acid phosphatase (AP) within the specific granules of eosinophils is held in a latent, inactive form prior to degranulation. Eosinophils responded to membrane stimuli with activation of AP throughout the granules of the cell, prior to degranulation. Activation occurred within the time required for fixation of the cells by glutaraldehyde. Whereas resting normal eosinophils demonstrated AP activity in 7.8 +/- 1.2 per cent of the granules, cells stimulated with opsonized staphylococci demonstrated activity in 78.9 +/- 5.0 per cent of the granules, and the amount of lead phosphate reactant deposited per active granule was considerably more intense in the stimulated than in the unstimulated cells. A similar stimulation of AP activity in eosinophils occurred with exposure to the surface-active agent phorbol myristate acetate or the calcium ionophore A23187. Activation was not altered by 5 microgram. per ml. of cytochalasin B or 4 x 10(-6) M colchicine. These aspects of the response closely parallel the stimulation of oxidative metabolism of eosinophils; however, the two were separable. Eosinophils obtained from a patient with chronic granulomatous disease, although unable to activate the normal oxidative metabolic burst, demonstrated a normal activation of AP.
嗜酸性粒细胞特定颗粒内的酸性磷酸酶（AP）在脱颗粒之前以潜在的无活性形式存在。在脱颗粒之前，嗜酸性粒细胞通过激活整个细胞颗粒中的AP来响应膜刺激。激活发生在戊二醛固定细胞所需的时间内。静止的正常嗜酸性粒细胞在7.8±1.2%的颗粒中表现出AP活性，而用调理过的葡萄球菌刺激的细胞在78.9±5.0%的颗粒中表现出活性，并且每个活性颗粒中沉积的磷酸铅反应物的量在受刺激的细胞中比未受刺激的细胞中明显更强烈。用表面活性剂佛波酯肉豆蔻酸酯乙酸盐或钙离子载体A23187处理嗜酸性粒细胞时，也会出现类似的AP活性刺激。5微克/毫升的细胞松弛素B或4×10⁻⁶ M的秋水仙碱不会改变激活作用。这种反应的这些方面与嗜酸性粒细胞氧化代谢的刺激密切平行；然而，两者是可分离的。从慢性肉芽肿病患者获得的嗜酸性粒细胞，尽管无法激活正常的氧化代谢爆发，但表现出正常的AP激活。
