An improved approach to histofluorescence using the SPG method for tissue monoamines.

A modification of the SPG histofluorescence method for the visualization of monoamines in tissues using cryostat sections is described. This modification has let to consistent standardization of the fluorescent intensity from one tissue section to another while also eliminating a cumbersome step in the procedure. The present procedure results in highly rapid and sensitive visualization of dopamine-, noradrenaline- and serotonin-containing neurons and their axonal varicosities. Processing time from fresh tissue to microscopic examination requires less than 15 min.