De la Torre J C
J Neurosci Methods. 1980 Oct;3(1):1-5. doi: 10.1016/0165-0270(80)90029-1.
A modification of the SPG histofluorescence method for the visualization of monoamines in tissues using cryostat sections is described. This modification has let to consistent standardization of the fluorescent intensity from one tissue section to another while also eliminating a cumbersome step in the procedure. The present procedure results in highly rapid and sensitive visualization of dopamine-, noradrenaline- and serotonin-containing neurons and their axonal varicosities. Processing time from fresh tissue to microscopic examination requires less than 15 min.
本文描述了一种对SPG组织荧光法的改进方法，该方法用于使用低温恒温器切片观察组织中的单胺。这种改进使得从一个组织切片到另一个组织切片的荧光强度能够持续标准化，同时也消除了该过程中一个繁琐的步骤。目前的方法能够快速且灵敏地观察到含多巴胺、去甲肾上腺素和5-羟色胺的神经元及其轴突膨体。从新鲜组织到显微镜检查的处理时间不到15分钟。
