Torre J C, Surgeon J W
Histochemistry. 1976 Oct 22;49(2):81-93. doi: 10.1007/BF00495672.
A modified approach of the glyoxylic acid (GA) condensation reaction for the visualization of biogenic amines in tissue is described. Cryostat sections are used from brain or extracerebral tissue in dog, monkey, rat and mouse and exposed for 3 s to a room temperature solution containing sucrose-potassium phosphate-glyoxylic acid (SPG). The tissues are air dried and heated in an oven for 5 min. The complete precessing time from fresh tissue to microscopic examineation takes 18 min. Morphologically sharp and brightly fluorescent monoamine-containing neurons, pre- and terminal axons are seen against a dark parenchymal background without drug pre-treatment. The SPG method retains the high specific sensitivity for monoamines previously described in the original technique but is, in addition, more rapid and simple and is easily accissible as a research tool to investigators inexperienced in histofluoresecence techniques.
本文描述了一种改良的乙醛酸（GA）缩合反应方法，用于组织中生物胺的可视化。使用来自狗、猴、大鼠和小鼠的脑或脑外组织的冷冻切片，将其暴露于含有蔗糖-磷酸钾-乙醛酸（SPG）的室温溶液中3秒。组织在空气中干燥并在烤箱中加热5分钟。从新鲜组织到显微镜检查的完整处理时间为18分钟。在未经药物预处理的情况下，在深色实质背景下可以看到形态清晰且荧光明亮的含单胺神经元、轴突前体和终末轴突。SPG方法保留了原始技术中先前描述的对单胺的高特异性敏感性，但此外，它更快速、简单，并且对于没有组织荧光技术经验的研究人员来说，作为一种研究工具很容易获得。
