Fan C C, Ho R J
Mol Cell Biochem. 1981 Jan 20;34(1):35-41. doi: 10.1007/BF02354850.
Hormone stimulated lipolysis of mouse and rabbit adipocytes as measured by both free fatty acid and glycerol release, is proportionally elevated with increase in the adipocyte cAMP level up to 1 nmol/g. The correlation coefficients are 0.94 and 0.97 for FFA/cAMP and glycerol/cAMP respectively. Increments in cAMP greater than 1 nmol/g show no correlation with increase in lipolysis. The release of lipolytic products, glycerol and free fatty acids, from white adipocytes in response to ACTH, epinephrine or norepinephrine was measured using radiochemical assays in short term incubation systems, with cAMP levels measured at the same time and from the same cell sample. Under the conditions studied, epinephrine is a more effective lipolytic hormone than ACTh in mouse adipocyte, and ACTH is more effective than epinephrine in rabbit adipocyte. The effect of catecholamines on the rabbit adipocyte is not modified by phentolamine (10 microM), but it is potentiated by 1-methyl-3-isobutyl xanthine (0.1 mM). The results suggest that cAMP mediates the action of these lipolytic hormones in white adipocytes of mouse and rabbit.
通过游离脂肪酸和甘油释放量来衡量，激素刺激的小鼠和兔脂肪细胞的脂解作用，随着脂肪细胞环磷酸腺苷（cAMP）水平升高至1 nmol/g而相应升高。游离脂肪酸（FFA）/cAMP和甘油/cAMP的相关系数分别为0.94和0.97。cAMP增量大于1 nmol/g时，与脂解作用的增加无相关性。在短期孵育系统中，使用放射化学分析法测定白色脂肪细胞对促肾上腺皮质激素（ACTH）、肾上腺素或去甲肾上腺素的反应中脂解产物甘油和游离脂肪酸的释放量，同时并从相同细胞样本中测量cAMP水平。在所研究的条件下，肾上腺素在小鼠脂肪细胞中是比ACTH更有效的脂解激素，而ACTH在兔脂肪细胞中比肾上腺素更有效。儿茶酚胺对兔脂肪细胞的作用不受酚妥拉明（10 microM）的影响，但可被1-甲基-3-异丁基黄嘌呤（0.1 mM）增强。结果表明，cAMP介导了这些脂解激素在小鼠和兔白色脂肪细胞中的作用。
