Wada M, Akanuma Y, Kimura N, Nagata N
Endocrinology. 1976 Jan;98(1):84-90. doi: 10.1210/endo-98-1-84.
The effects of ACTH on 3',5'-cyclic AMP (cAMP) levels and lipolysis were examined on isolated adipocytes incubated in either isosmolar or hyperosmolar media. The ability of ACTH to induce intracellular cAMP accumulation was greatly enhanced by incubating cells in hyperosmolar sucrose (100 to 400 mM) solutions. Hyperosmolar solutions prepared by the addition of either NaCL, glucose or mannitol enhanced the ACTH effect on cAMP to the same extent as did the hyperosmolar sucrose solution, but hyperosmolar urea solutions did not have such an effect. The effect of hyperosmolarity was shown only in cells stimulated by lipolytic hormones, and the effects were still evident in the presence of high concentrations of theophylline, indicating the effect of hyperosmolarity is to facilitate hormone action on the receptor-coupler system of the adipocyte membrane. The action of glucagon on cAMP was augmented much less than the actions of ACTH and isoproterenol. Basal as well as ACTH or exogenous cAMP stimulated lipolysis was lower in hyperosmolar sucrose solutions. Some mechanism by which hyperosmolarity interferes with the metabolic sequence beyond the accumulation of cAMP was suggested.
在等渗或高渗培养基中孵育的分离脂肪细胞上，研究了促肾上腺皮质激素（ACTH）对3',5'-环磷酸腺苷（cAMP）水平和脂肪分解的影响。通过在高渗蔗糖（100至400 mM）溶液中孵育细胞，ACTH诱导细胞内cAMP积累的能力大大增强。通过添加NaCl、葡萄糖或甘露醇制备的高渗溶液对ACTH对cAMP的作用增强程度与高渗蔗糖溶液相同，但高渗尿素溶液没有这种作用。高渗作用仅在脂解激素刺激的细胞中显示，并且在高浓度茶碱存在下作用仍然明显，表明高渗作用是促进激素对脂肪细胞膜受体偶联系统的作用。胰高血糖素对cAMP的作用增强程度远小于ACTH和异丙肾上腺素。在高渗蔗糖溶液中，基础以及ACTH或外源性cAMP刺激的脂肪分解较低。有人提出了高渗干扰cAMP积累后代谢序列的某种机制。
