Stürmer C, Bielenberg K, Spatz W B
Neurosci Lett. 1981 Apr 9;23(1):1-5. doi: 10.1016/0304-3940(81)90181-6.
Tetramethylbenzidine (TMB) histochemistry for retrogradely transported horseradish peroxidase (HRP) has been successfully applied in electron microscopical preparations in the guinea pig visual cortex. In ultrathin sections, the TMB--HRP reaction product appears as an accumulation of typical electron-dense crystals. These crystals were identified in the cytoplasm of labeled cells in the proximal portions of the dendrites, and in myelinated axons. It is demonstrated that the TMB--HRP method is well suitable to analyze the ultrastructure of labeled cells and their synaptology.
用于逆行转运辣根过氧化物酶（HRP）的四甲基联苯胺（TMB）组织化学已成功应用于豚鼠视皮层的电子显微镜制备中。在超薄切片中，TMB - HRP反应产物表现为典型的电子致密晶体的聚集。这些晶体在树突近端标记细胞的细胞质以及有髓轴突中被鉴定出来。结果表明，TMB - HRP方法非常适合分析标记细胞的超微结构及其突触学。
