Svensson B A, Rastad J, Westman J
Exp Brain Res. 1984;55(2):325-32. doi: 10.1007/BF00237283.
Endogenous peroxidase-like activity was investigated with a combined light and electron microscopical technique in 15 cats. The lateral cervical nucleus, the dorsal column nuclei, and segments C6 and L5 of the spinal cord were incubated with diaminobenzidine-tetrahydrochloride (DAB) or tetramethylbenzidine (TMB). After histochemical reaction with DAB a considerable amount of activity was found in nerve cells, astrocytes and pericytes. The neuronal labelling was mainly located in mitochondria of axon terminals and in dendrites whereas the astrocytic and pericytic activity was found in cytoplasmic dense bodies. The quantity of stained structures differed considerably between the animals. In TMB reacted tissue endogenous peroxidase-like activity was only sparsely seen. It was found mainly in frozen sections, in which the neuropil and perivascular structures sometimes contained granules and irregular filaments. The significance of the findings is discussed in relation to observations in tracer studies using horseradish peroxidase.
采用光镜和电镜联合技术，对15只猫的内源性过氧化物酶样活性进行了研究。将颈外侧核、薄束核以及脊髓颈6和腰5节段用二氨基联苯胺四盐酸盐（DAB）或四甲基联苯胺（TMB）进行孵育。用DAB进行组织化学反应后，在神经细胞、星形胶质细胞和周细胞中发现了相当数量的活性。神经元标记主要位于轴突终末的线粒体和树突中，而星形胶质细胞和周细胞的活性则见于细胞质致密小体中。不同动物之间染色结构的数量差异很大。在TMB反应的组织中，仅偶尔可见内源性过氧化物酶样活性。主要在冰冻切片中发现，其中神经纤维网和血管周围结构有时含有颗粒和不规则细丝。结合使用辣根过氧化物酶的示踪研究中的观察结果，对这些发现的意义进行了讨论。
