Troyer H, Babich E
Stain Technol. 1981 Jan;56(1):39-43. doi: 10.3109/10520298109067273.
A staining procedure is described for use with glycol methacrylate embedded tissue sections which does not stain the plastic embedment or remove the sections from the glass slides. The basic dye is celestine blue B. It is prepared by treating 1 g of the dye with 0.5 ml concentrated sulfuric acid. It is then dissolved with the following solution. Add 14 ml glycerine to 100 ml 2.5% ferric ammonium sulfate and warm the solution to 50 C. Finally adjust the pH to 0.8 to 0.9 The acid staining solution consists of 0.075% ponceau de xylidine and 0.025% acid fuchsin in 10% acetic acid. Slides containing the dried plastic sections are immersed in the celestine blue solution for five minutes and in the ponceau-fuchsin solution for ten minutes with an intervening water rinse. After a final wash, the sections are air dried and coverslipped. This staining procedure colors the tissues nearly the same as hematoxylin and eosin procedures.
本文描述了一种用于甲基丙烯酸乙二醇酯包埋组织切片的染色方法，该方法不会使塑料包埋剂染色，也不会将切片从载玻片上取下。碱性染料为天青石蓝B。其制备方法是将1克染料与0.5毫升浓硫酸混合处理，然后用以下溶液溶解。将14毫升甘油加入100毫升2.5%硫酸铁铵中，将溶液加热至50℃，最后将pH值调至0.8至0.9。酸性染色液由0.075%二甲苯苯胺红紫和0.025%酸性品红溶于10%乙酸中组成。将含有干燥塑料切片的载玻片浸入天青石蓝溶液中5分钟，再浸入二甲苯苯胺红紫-酸性品红溶液中10分钟，中间用水冲洗。最后洗涤后，将切片空气干燥并盖上盖玻片。这种染色方法使组织的染色效果与苏木精和伊红染色法几乎相同。
