[Diagnostic value of the protein antigens of Br. abortus and Yersinia enterocolitica type 9].

Protein antigens were obtained from Br. abortus 99 and Yersinia enterocolitica, type 9, and were titered on guinea-pigs infected with a living culture from these microbes. It was proved that the guinea-pigs infected with Yersinia reacted with a skin-allergic reaction only to an antigen obtained from Yersinia and not from Brucella. Conversely, the animals infected with Br. abortus B-19 reacted during skin treatment only to an allergen from Brucella, but not with an allergen from Yersinia. In a rabbit anti-yersinia serum were proved precipitins through the immunodiffusion test against the protein allergen from Yersinia, whereas no Brucella precipitins were detected with a protein allergen. In anti-Brucella rabbit serum (Brucella amboceptor or agglutinative serum, no precipitins were proved with Brucella and Yersinia protein allergens. In a hiperimmune rabbit serum against Brucella and Yersinia were proved precipitins against a polysaccharide both from Yersinia and Brucella. It was adopted that protein antigens in the case of Brucella and Yersinia were specific and that specificity could be used for differentiation of these two infections.