Nozicková M, Bartos V, Hachova L
Z Exp Chir. 1981 Feb;14(1):30-4.
In 14 dogs pancreatectomy was performed and pancreas was submitted to 24 hour hypothermic pulsatile perfusion using Gambro machine. During the perfusion exocrine secretion subsequent to secretin and CCK stimulation was collected and the perfusion fluid was analyzed. In 7 of these animals, Venalot, a combination of coumarin and rutin sulphate, was added to the perfusion fluid and its effect was tested. The addition of Venalot was followed by a decreased vascular peripheral resistance, decreased weight of the perfused graft, and decreased level of LDH and lactic acid in the perfusion fluid. The release of potassium from the pancreatic cells was prevented. The parameters of the pancreatic secretion was better than in the control group. Benzo-pyrones reduced oedema formation and improved cell metabolism during hypothermic perfusion of the pancreas.
对14只狗实施了胰腺切除术，并使用 Gambro 机器对胰腺进行了24小时的低温搏动灌注。在灌注过程中，收集了促胰液素和胆囊收缩素刺激后的外分泌分泌物，并对灌注液进行了分析。在其中7只动物中，将香豆素和硫酸芦丁的组合 Venalot 添加到灌注液中并测试其效果。添加 Venalot 后，血管外周阻力降低，灌注移植物重量减轻，灌注液中乳酸脱氢酶和乳酸水平降低。胰腺细胞的钾释放得到了抑制。胰腺分泌参数优于对照组。苯并吡喃在胰腺低温灌注期间减少了水肿形成并改善了细胞代谢。
