Pagnini C A, Bozzola L
Ann Anat Pathol (Paris). 1980;25(3):261-5.
A report concerning the histochemical methods used to differentiate mucosubstances and certain technical modifications made with the aim of obtaining better results : 1) rapid passage in a solution at pH1 after alcian blue reaction in order to ensure staining of sulfate mucosubstances only ; 20 plunging of sections first in FeCl for 10 minutes by the H.I.D. method ; 3) in the methylation reaction the optimum time is 12 hours.
1）在阿尔辛蓝反应后快速通过pH1的溶液，以确保仅对硫酸黏液物质进行染色；2）通过高铁二胺法将切片先浸入氯化铁中10分钟；3）在甲基化反应中，最佳时间为12小时。
