de Waal L P, Melief C J, Melvold R W
Eur J Immunol. 1981 Mar;11(3):258-65. doi: 10.1002/eji.1830110317.
The recently discovered B6.C-H-2bm12 (bm12) histocompatibility mutant bears a mutation of the I-A subregion of the H-2b haplotype. We have studied cytotoxic T lymphocytes (CTL) generated in secondary mixed lymphocyte culture (MLC) between the bm12 mutant and the strain of origin C57BL/6 (B6). In both directions, strong CTL responses were generated against lipopolysaccharide-stimulated, but not against concanavalin A-stimulated target cells. Studies of the specificity of the bm12 anti-B6 CTL response, using target cells from a panel of H-2 recombinants, showed that the CTL were directed against specificities of the I-Ab subregion. This conclusion was confirmed by the observation that the bm12 anti-B6 CTL could be specifically blocked by anti-Iab, but not by anti-Kb antisera. EL4 tumor cells, which express Kb and Db but not Iab antigens, were not lysed by the CTL. The combined data confirm that bm12 is an I-A subregion mutant and indicate that the I-A subregion antigens, recognized by antibody and those recognized by CTL, are probably present on the same molecules. The CTL effector cells generated in secondary MLC across the I-Ab mutant difference had the surface phenotype Lyt 1- 2+.
最近发现的B6.C-H-2bm12（bm12）组织相容性突变体带有H-2b单倍型I-A亚区的突变。我们研究了在bm12突变体与亲本菌株C57BL/6（B6）之间的二次混合淋巴细胞培养（MLC）中产生的细胞毒性T淋巴细胞（CTL）。在两个方向上，针对脂多糖刺激的靶细胞产生了强烈的CTL反应，但对刀豆蛋白A刺激的靶细胞没有产生反应。使用一组H-2重组体的靶细胞对bm12抗B6 CTL反应的特异性进行研究，结果表明CTL针对的是I-Ab亚区的特异性。抗Iab抗体可特异性阻断bm12抗B6 CTL，而抗Kb抗血清则不能，这一观察结果证实了该结论。表达Kb和Db但不表达Iab抗原的EL4肿瘤细胞未被CTL裂解。综合数据证实bm12是I-A亚区突变体，并表明抗体识别的I-A亚区抗原和CTL识别的抗原可能存在于同一分子上。在跨越I-Ab突变差异的二次MLC中产生的CTL效应细胞具有Lyt 1- 2+的表面表型。
