Nepom J T, Benacerraf B, Germain R N
J Immunol. 1981 Jul;127(1):31-4.
T cell proliferative responses to the synthetic polypeptides GAT and GLPhe are under Ir gene control. GAT responses are regulated by gene(s) in the I-A subregion, and GLPhe responses are controlled by a pair of complementing genes mapping to the I-A and I-E subregions. We demonstrate that monoclonal antibody to the I-A gene product inhibits GAT proliferation but not the GLPhe response, whereas a monoclonal antibody to the I-E associated Ia-7 determinant inhibits GLPhe but not GAT proliferation, which indicates independent involvement of each Ia determinant in antigen presentation for the T cell response to these antigens. Use of the same subregion-specific monoclonal antibodies in complement-dependent lysis demonstrates that the antigen-presenting cells for GAT and GLPhe express both I-A and I-E products. The possibility that an Ia subregion-specific "self-receptor" functions on the reactive T cells as a regulatory element is discussed.
T细胞对合成多肽GAT和GLPhe的增殖反应受Ir基因控制。GAT反应受I-A亚区基因调控，而GLPhe反应由一对定位于I-A和I-E亚区的互补基因控制。我们证明，针对I-A基因产物的单克隆抗体可抑制GAT增殖，但不抑制GLPhe反应，而针对与I-E相关的Ia-7决定簇的单克隆抗体可抑制GLPhe反应，但不抑制GAT增殖，这表明每个Ia决定簇在这些抗原的T细胞反应的抗原呈递中独立发挥作用。在补体依赖性裂解中使用相同的亚区特异性单克隆抗体表明，GAT和GLPhe的抗原呈递细胞同时表达I-A和I-E产物。文中还讨论了Ia亚区特异性“自身受体”作为调节元件在反应性T细胞上发挥作用的可能性。
