Schook L B, Allen P M, Niederhuber J E
J Immunol. 1983 Feb;130(2):661-4.
Marrow stem cells cultured with L cell-conditioned medium were used to produce large numbers of macrophages free of contaminating lymphoid cells or granulocytes. Experiments were performed by using these bone marrow-derived macrophages (BMDM) as antigen-presenting cells (APC) for the terpolymer antigen L-glutamic60-L-alanine30-L-tyrosine10 (GAT). By using antigen-specific T cell proliferation, it was demonstrated that BMDM were equal to splenic macrophages in their capacity to present GAT. Furthermore, when BMDM were pretreated with alloantibodies specific for Ia antigens of the I-A subregion, the T cell proliferative response to GAT was inhibited. The I-A subregion is known to be the site of Ir gene regulation of the GAT response, and antibodies to other subregions had no inhibitory effect. Monoclonal antibodies recognizing a beta-chain product of the I-A subregion (Ia. 17) also inhibited the BMDM from effective antigen presentation. These results were similar to those obtained with purified splenic macrophages.
用L细胞条件培养基培养的骨髓干细胞用于大量生产不含污染淋巴细胞或粒细胞的巨噬细胞。实验通过使用这些骨髓来源的巨噬细胞（BMDM）作为三元聚合物抗原L-谷氨酸60-L-丙氨酸30-L-酪氨酸10（GAT）的抗原呈递细胞（APC）来进行。通过使用抗原特异性T细胞增殖，证明BMDM在呈递GAT的能力上与脾巨噬细胞相当。此外，当BMDM用针对I-A亚区Ia抗原的同种抗体预处理时，对GAT的T细胞增殖反应受到抑制。已知I-A亚区是GAT反应的Ir基因调控位点，针对其他亚区的抗体没有抑制作用。识别I-A亚区β链产物（Ia.17）的单克隆抗体也抑制BMDM进行有效的抗原呈递。这些结果与用纯化的脾巨噬细胞获得的结果相似。
