Goff S, Traktman P, Baltimore D
J Virol. 1981 Apr;38(1):239-48. doi: 10.1128/JVI.38.1.239-248.1981.
A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Moloney murine leukemia virus have been isolated which (i) produce a thermolabile reverse transcriptase, (ii) are temperature sensitive for release of enzyme activity, or (iii) can only productively infect cells already producing gag-related polypeptides. The assay has also been useful for the isolation of nonproducer cells infected with various replication-defective transforming viruses.
我们开发了一种快速检测方法，用于检测受感染细胞释放到培养基中的逆转录病毒逆转录酶活性。通过该检测方法，可在数小时内对4000个克隆感染的细胞系进行检测。我们已对该检测方法进行了改进，将其用作筛选自发病毒突变体的手段。现已分离出莫洛尼鼠白血病病毒的突变体，这些突变体：（i）产生热不稳定的逆转录酶；（ii）在酶活性释放方面对温度敏感；或（iii）只能有效感染已产生与gag相关多肽的细胞。该检测方法对于分离感染各种复制缺陷型转化病毒的非生产性细胞也很有用。
