Lai M M, Stohlman S A
J Virol. 1981 May;38(2):661-70. doi: 10.1128/JVI.38.2.661-670.1981.
The RNA genomes of various murine hepatitis virus (MHV) strains were studied by T1-oligonucleotide fingerprinting analysis with regard to their structure and sequence relationship. It was found that the MHV particles contained only positive-stranded 60S RNA which had a "cap" structure at its 5' end. No negative-stranded RNA was found. It was also shown that most of the MHV strains had diverged quite extensively in their genetic sequences. However, MHV-3, a hepatotropic strain, and A59, a nonpathogenic strain, were found to have very similar oligonucleotide fingerprinting patterns. Yet, each of them contained two to four specific oligonucleotides. The MHV-3-specific oligonucleotides were conserved in almost all of the hepatotropic MHV strains studied. In contrast, two of the A59-specific oligonucleotides were absent from the genomes of all hepatotropic strains. These findings suggest that these unique oligonucleotides might be localized at the genetic region(s) associated with viral pathogenicity or other biological properties of the virus. Comparison of viral structural proteins also suggests that MHV-3 and A59 are more closely related than other MHV strains. The significance of these findings is discussed.
通过T1 - 寡核苷酸指纹图谱分析，研究了各种鼠肝炎病毒（MHV）毒株的RNA基因组，以了解它们的结构和序列关系。结果发现，MHV颗粒仅含有在其5'端具有“帽”结构的正链60S RNA。未发现负链RNA。研究还表明，大多数MHV毒株在其基因序列上有很大程度的差异。然而，发现嗜肝毒株MHV - 3和非致病性毒株A59具有非常相似的寡核苷酸指纹图谱模式。不过，它们各自都含有两到四个特定的寡核苷酸。MHV - 3特异性寡核苷酸在几乎所有研究的嗜肝MHV毒株中都是保守的。相比之下，所有嗜肝毒株的基因组中都不存在A59特异性寡核苷酸中的两个。这些发现表明，这些独特的寡核苷酸可能位于与病毒致病性或病毒其他生物学特性相关的基因区域。病毒结构蛋白的比较也表明，MHV - 3和A59比其他MHV毒株关系更密切。讨论了这些发现的意义。
