纯化呼肠孤病毒体外合成甲基化信使核糖核酸
Methylated messenger RNA synthesis in vitro by purified reovirus.
作者信息
Shatkin A J
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3204-7. doi: 10.1073/pnas.71.8.3204.
Abstract
Purified human reovirus contains RNA methylase activity in addition to an RNA polymerase. Virions incubated under appropriate conditions in the presence of S-adenosyl-L-methionine synthesize mRNA that is specifically methylated in the 5'-terminal guanosine. Alkaline digestion of the methylated RNA released a 5'-terminal dinucleotide, ppG'pCp, indicating that the guanosine contains 2'-O-methylribose. The possible roles of methylation in viral and cellular mRNA function is discussed.
摘要
纯化的人呼肠孤病毒除了含有RNA聚合酶外，还具有RNA甲基化酶活性。在适当条件下，病毒粒子在S-腺苷-L-甲硫氨酸存在下孵育，合成在5'-末端鸟苷处发生特异性甲基化的mRNA。对甲基化RNA进行碱性消化，释放出5'-末端二核苷酸ppG'pCp，表明鸟苷含有2'-O-甲基核糖。本文讨论了甲基化在病毒和细胞mRNA功能中的可能作用。
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本文引用的文献
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"Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA.含双链RNA的细胞质多角体病毒的病毒相关转录酶的“甲基化偶联”转录
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