Kurtz D T
Nature. 1981 Jun 25;291(5817):629-31. doi: 10.1038/291629a0.
Two different genes coding for the hormonally regulated rat liver protein alpha 2u globulin were introduced into mouse Ltk- cells through co-transfection with the HSV-1 thymidine kinase gene. Three to ten copies of the alpha 2u globulin genes were detected several tk+ clones, over 50% of which could be induced with dexamethasone, the produce alha 2u globulin mRNA and protein. This suggests that the information necessary for hormonal response is contained in the DNA fragment used for transfer.
通过与单纯疱疹病毒1型胸苷激酶基因共转染，将编码激素调节的大鼠肝脏蛋白α2u球蛋白的两种不同基因导入小鼠Ltk-细胞。在几个tk+克隆中检测到三到十个拷贝的α2u球蛋白基因，其中超过50%可被地塞米松诱导，产生α2u球蛋白mRNA和蛋白质。这表明激素反应所需的信息包含在用于转移的DNA片段中。
