[多粘芽孢杆菌N3中β-淀粉酶的分离与特性研究]
[Isolation and characterization of beta-amylase from Bacillus polymyxa N3].
作者信息
Rashap R K, Iushkaĭte Z A, Glemzha A A
出版信息
Prikl Biokhim Mikrobiol. 1981 Mar-Apr;17(2):225-32.
The effect of cultivation conditions of Bacillus polymyxa N 3 on the intensity of beta-amylase accumulation during cultivation, procedure of isolation and 100-fold purification as well as physico-chemical properties was investigated. From the culture medium the enzyme was isolated by ethanol precipitation. The highly purified enzyme was obtained by corn starch chromatography. The molecular weight of the PAAG homogeneous preparation was estimated to be 48,000. The optimum temperature of the enzyme reaction was 50 degrees C. The rate of soluble starch hydrolysis by beta-amylase was found to depend on pH.
研究了多粘芽孢杆菌N 3的培养条件对培养过程中β-淀粉酶积累强度、分离及100倍纯化过程以及理化性质的影响。通过乙醇沉淀从培养基中分离出该酶。通过玉米淀粉色谱法获得了高度纯化的酶。聚丙二醇（PAAG）均一制剂的分子量估计为48,000。该酶反应的最佳温度为50℃。发现β-淀粉酶水解可溶性淀粉的速率取决于pH值。
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