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多粘芽孢杆菌β-淀粉酶基因的克隆与特性分析

Cloning and characterization of the beta-amylase gene from Bacillus polymyxa.

作者信息

Friedberg F, Rhodes C

出版信息

J Bacteriol. 1986 Mar;165(3):819-24. doi: 10.1128/jb.165.3.819-824.1986.

DOI:10.1128/jb.165.3.819-824.1986
PMID:2419310
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214501/
Abstract

The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydrolysis with this beta-amylase produces maltose, not glucose, whereas maltotriose and cycloheptaose are resistant to the action of this beta-amylase. The enzyme has a molecular weight of approximately 68,000. Restriction endonuclease mapping demonstrated that the DNA inserted in pBD64 and containing the gene is approximately 3 kilobases in length.

摘要

通过在枯草芽孢杆菌中进行分子克隆，从多粘芽孢杆菌中分离出β-淀粉酶基因。含有该基因的枯草芽孢杆菌细胞表达并分泌一种淀粉酶，这种淀粉酶在碳水化合物水解过程中产生的产物方面，类似于多粘芽孢杆菌β-淀粉酶和大麦β-淀粉酶。用这种β-淀粉酶进行淀粉水解产生的是麦芽糖，而非葡萄糖，而麦芽三糖和环庚糖对这种β-淀粉酶的作用具有抗性。该酶的分子量约为68,000。限制性内切酶图谱分析表明，插入pBD64中并包含该基因的DNA长度约为3千碱基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/c3c6fd61b1bb/jbacter00214-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/54e480899ef8/jbacter00214-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/74a668bfff07/jbacter00214-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/36a62101e907/jbacter00214-0167-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/1416864508e9/jbacter00214-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/55068eaad39e/jbacter00214-0168-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/c3c6fd61b1bb/jbacter00214-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/54e480899ef8/jbacter00214-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/74a668bfff07/jbacter00214-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/36a62101e907/jbacter00214-0167-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/1416864508e9/jbacter00214-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/55068eaad39e/jbacter00214-0168-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83a/214501/c3c6fd61b1bb/jbacter00214-0169-a.jpg

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本文引用的文献

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J Bacteriol. 1989 Jan;171(1):375-82. doi: 10.1128/jb.171.1.375-382.1989.
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Cloning and sequencing of the gene encoding thermophilic beta-amylase of Clostridium thermosulfurogenes.嗜热栖热硫化叶菌嗜热β-淀粉酶编码基因的克隆与测序
J Bacteriol. 1988 Dec;170(12):5848-54. doi: 10.1128/jb.170.12.5848-5854.1988.
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Sequence of an active fragment of B. polymyxa beta amylase.多粘芽孢杆菌β淀粉酶活性片段的序列
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Cloning and nucleotide sequence of the gene coding for enzymatically active fragments of the Bacillus polymyxa beta-amylase.多粘芽孢杆菌β-淀粉酶酶活性片段编码基因的克隆及核苷酸序列
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