Longo D L, Schwartz R H
Proc Natl Acad Sci U S A. 1981 Jan;78(1):514-8. doi: 10.1073/pnas.78.1.514.
Chimeric B10.A T cells that had matured in a (B10.A X B10.Q)F1 environment acquired the ability to respond to poly(Glu56Lys35Phe9) (GL pi), an antigen to which the B10.A mouse is a nonresponder. The response of the chimeric B10.A T cells was initiated by GL phi on responder B10.Q antigen-presenting cells (APC) but not by GL phi on nonresponder B10.A APC. Similarly, chimeric B10.Q T cells that had matured in a (B10.A X B10.Q)F1 environment acquired the ability to respond to poly(Glu60Ala30Tyr10) (GAT) when the antigen was presented on responder B10.A APC, but not when GAT was presented on nonresponder B10.Q APC. No syngeneic haplotype preference was observed for either antigen. These interactions between H-2 nonidentical T cells and APC were inhibited by anti-H-2 antisera and a monoclonal anti-Ia antibody directed against the APC but not by such antibodies when they were directed against the T cell. These data suggest that, when they develop in a responder chimeric environment, genotypic nonresponder T cells become responders by acquiring receptors that allow them to recognize responder I region products on the surface of APC. Furthermore, the data demonstrate that the site of action of the blocking effects of the anti-Ia antibodies is the APC, thus providing strong evidence in support of the idea that Ia antigens on APC are the Ir gene products.
在（B10.A×B10.Q）F1环境中成熟的嵌合B10.A T细胞获得了对多聚（Glu56Lys35Phe9）（GL pi）产生应答的能力，GL pi是B10.A小鼠无应答的一种抗原。嵌合B10.A T细胞的应答由应答性B10.Q抗原呈递细胞（APC）上的GL phi启动，而非应答性B10.A APC上的GL phi则不能启动。同样，在（B10.A×B10.Q）F1环境中成熟的嵌合B10.Q T细胞，当抗原由应答性B10.A APC呈递时，获得了对多聚（Glu60Ala30Tyr10）（GAT）产生应答的能力，但当GAT由非应答性B10.Q APC呈递时则不能产生应答。两种抗原均未观察到同基因单倍型偏好。H-2不相同的T细胞与APC之间的这些相互作用被抗H-2抗血清和针对APC的单克隆抗Ia抗体所抑制，但当这些抗体针对T细胞时则无此抑制作用。这些数据表明，当基因型无应答T细胞在应答性嵌合环境中发育时，通过获得能够识别APC表面应答性I区产物的受体而成为应答细胞。此外，数据表明抗Ia抗体阻断作用的作用位点是APC，从而为APC上的Ia抗原是Ir基因产物这一观点提供了有力证据。
