Trisler G D, Schneider M D, Nirenberg M
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2145-9. doi: 10.1073/pnas.78.4.2145.
A monoclonal antibody was obtained that binds to cell membrane molecules distributed in a topographic gradient in avian retina. Thirty-five-fold more antigen was detected in dorsoposterior retina that in ventroanterior retina. Most of the antigen was associated with the synaptic layers of the retina. Less antigen was detected in cerebrum, thalamus, cerebellum, and optic tectum, but little or none was found in non-neural tissues tested. The antigen was found on most or all cell types in retina, and the concentration of antigen found is a function of the square of the circumferential distance from the ventroanterior pole of the gradient toward the dorsoposterior pole. Thus, the antigen can be used as a marker of cell position along the ventroanterior-dorsoposterior axis of the retina.
获得了一种单克隆抗体，它能与在鸟类视网膜中呈拓扑梯度分布的细胞膜分子结合。在视网膜背后部检测到的抗原比腹前部多35倍。大部分抗原与视网膜的突触层相关。在大脑、丘脑、小脑和视顶盖中检测到的抗原较少，但在所测试的非神经组织中几乎没有或未检测到抗原。在视网膜的大多数或所有细胞类型上都发现了这种抗原，并且所发现的抗原浓度是从梯度的腹前极向背后极的圆周距离平方的函数。因此，该抗原可作为沿视网膜腹前 - 背后轴细胞位置的标记物。
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