Barbera A J, Marchase R B, Roth S
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2482-6. doi: 10.1073/pnas.70.9.2482.
An assay has been developed to test the hypothesis that neurons from a limited area of the retina will adhere preferentially to that part of the optic tectum near their normal synaptic termini. The method measures the adherence of (32)P-labeled cell bodies from either the dorsal or ventral half of the neural retina of chick embryos to dorsal and ventral tectal halves. When a labeled, single-cell suspension is prepared from dorsal half-retina, more cells bind to the ventral half of the tectum. When the labeled cells are from ventral half-retina, more bind to dorsal half-tecta. This preferential adhesion mimics the retinotectal projection found in vivo. Dorsal retinal cells show this preference shortly after dissociation with crude trypsin, and maintain it for at least 9 hr. Ventral retinal cells, however, require incubation in nutrient medium after trypsinization in order to display this selectivity. Comparable results are obtained when the cell suspension is prepared from pigmented retina. The data support an interpretation of neuronal specificity dependent on cell-surface interactions and demonstrate a clear correlation between selective adhesion and biological function.
来自视网膜有限区域的神经元将优先附着于视顶盖中靠近其正常突触终末的部分。该方法测量来自鸡胚神经视网膜背侧或腹侧半部分的（32）P标记细胞体与顶盖背侧和腹侧半部分的附着情况。当从背侧半视网膜制备标记的单细胞悬液时，更多细胞与顶盖腹侧半部分结合。当标记细胞来自腹侧半视网膜时，更多细胞与顶盖背侧半部分结合。这种优先附着模拟了体内发现的视网膜顶盖投射。背侧视网膜细胞在与粗胰蛋白酶解离后不久就表现出这种偏好，并至少维持9小时。然而，腹侧视网膜细胞在胰蛋白酶消化后需要在营养培养基中孵育才能表现出这种选择性。当从色素视网膜制备细胞悬液时，可获得类似结果。这些数据支持了一种依赖于细胞表面相互作用的神经元特异性解释，并证明了选择性附着与生物学功能之间存在明显的相关性。
