Shimamura T, Sasadaira H, Tsuru S, Zinnaka Y, Hashimoto K, Sasaki S
Tokai J Exp Clin Med. 1980 Oct;5(4):443-9.
Previous work has shown that normal mice respond to the intravenous injection of sheep erythrocytes (SRBC) with a transient increase in splenic cyclic AMP peaking after 2 minutes, and this cyclic AMP response has been shown to be due to prostaglandins (PGs) released from antigen-specific cells. In the present study, the cell type responsible for PG production in response to antigen was determined using a fluorescence-activated cell sorter (FACS) and an electron microscope. C57Bl/6 mice injected with SRBC showed a 2% increase of PGE-producing spleen cells relative to controls. In control mice injected with phosphate-buffered saline (PBS), 19% of the spleen cells produced PGE. An electron microscopic study of PGE-producing cells sorted on FACS showed that they were small lymphocytes and plasma cells. In addition, nude mice also increased the number of PGE-producing spleen cells in response to antigen. These findings suggest that lymphocytes involving at least B-cells respond to antigen with an increase in PGE production.
先前的研究表明，正常小鼠静脉注射绵羊红细胞（SRBC）后，脾脏中环磷酸腺苷（cAMP）会短暂升高，在2分钟时达到峰值，且这种cAMP反应已被证明是由抗原特异性细胞释放的前列腺素（PGs）所致。在本研究中，使用荧光激活细胞分选仪（FACS）和电子显微镜确定了响应抗原产生PG的细胞类型。注射SRBC的C57Bl/6小鼠与对照组相比，产生PGE的脾细胞增加了2%。在注射磷酸盐缓冲盐水（PBS）的对照小鼠中，19%的脾细胞产生PGE。对通过FACS分选的产生PGE的细胞进行电子显微镜研究表明，它们是小淋巴细胞和浆细胞。此外，裸鼠对抗原的反应也是产生PGE的脾细胞数量增加。这些发现表明，至少涉及B细胞的淋巴细胞对抗原的反应是PGE产生增加。
