[枸橼酸盐法从宋内志贺氏菌中提取抗原。I. 与其他方法的比较]

[Citrate method of extracting antigens from Shigella sonnei. I. Comparison with other methods].

作者信息

Kamzolkina N B, Iarovaia L M

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1981 Feb(2):72-7.

Abstract

The comparative characteristic of extracts obtained from Sh. sonnei by the method of Raynaud and Digeon and neurotoxin obtained by sedimentation with trichloroacetic acid from chloroform autolysates by the method of Mesrobeanu et al. is presented. Both preparations were shown to be similar in their antigenic structure and immunogenicity for mice and guinea-pigs. Nevertheless the extracts obtained by the citrate method contained considerably less protein and endotoxin and had no enterotoxic activity. This allows to recommend the citrate method for extracting protective antigens from Sh. sonnei.

摘要

本文介绍了用雷诺和迪让方法从宋内志贺菌中提取的提取物与用梅斯罗贝亚努等人的方法从氯仿自溶物中用三氯乙酸沉淀获得的神经毒素的比较特性。结果表明，这两种制剂对小鼠和豚鼠的抗原结构和免疫原性相似。然而，用柠檬酸盐法获得的提取物蛋白质和内毒素含量明显较低，且无肠毒素活性。这使得可以推荐用柠檬酸盐法从宋内志贺菌中提取保护性抗原。