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[宋内志贺菌抗原结构的免疫电泳分析]

[Immunoelectrophoretic analysis of the antigenic structure of Sh. sonnei].

作者信息

Belaia Iu A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1978 Jun(6):55-61.

PMID:79280
Abstract

The authors studied the antigenic composition of 105 Sh. sonnei strains freshly isolated from patients suffering from acute dysentery and carriers. Immunophoregrams of pure S-and R-forms species were obtained. Up to 13 antigens differing by electrophoretic and diffusion mobility and immunological specificity were revealed among soluble Sh. sonnei antigens The position of common and specific antigens was determined on the immunophoregram. Along with the thermostable somatic O-antigen detected at the I phase of the S-forms, and two thermolabile O-antigen components at the II phase, and the R-forms, there was revealed a surface, relatively thermolabile, K-antigen of A-type capable of agglutinating live bacteria in the O-antiserum; position of the latter on the immunophoregram was also determined.

摘要

作者研究了从急性痢疾患者和携带者中新鲜分离出的105株宋内志贺菌的抗原组成。获得了纯S型和R型菌株的免疫电泳图谱。在宋内志贺菌可溶性抗原中发现了多达13种在电泳和扩散迁移率以及免疫特异性方面存在差异的抗原。在免疫电泳图谱上确定了共同抗原和特异性抗原的位置。除了在S型菌株的I期检测到的耐热菌体O抗原、II期的两种不耐热O抗原成分以及R型菌株外,还发现了一种表面相对不耐热的A型K抗原,它能够在O抗血清中凝集活细菌;还确定了后者在免疫电泳图谱上的位置。

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